Microbial Diversity in Post-Mining Land in Makroman Kelurahan, Sambutan District Samarinda City

Abstract

East Kalimantan is one of the areas rich in mining land, one of which is coal. Coal mining activities in Kaltim are generally carried out with open mining techniques, namely by clearing land, peeling top soil, moving the top layer of soil, and then mining coal. This technique results in physical, chemical and biological damage to the soil, making it unsuitable for plant growth because it is poor in nutrients. The purpose of this study was to identify the available microorganisms in the soil of ex-coal mining. Soil sampling was carried out at the post-mining area in Makroman Village, Sambutan District, Samarinda City. Soil samples were taken with a depth of ± 30 cm. Soil is taken ± weighing 1 kg. Then put it in a plastic bag and label it. The soil sample is then taken to the laboratory. The media used were Potato Dextrose Agar media for culturing fungi and Nutrien Agar media for culturing bacteria. The nematodes were identified using extraction of soil samples used was the Baermann funnel method. The results showed that the types of fungi obtained based on the results of the study contained 4 (four) different types of fungi, namely Trichoderma sp., Penicillium sp., Fusarium sp., and Aspergillus sp.; There are two types of bacteria, the first is round bacteria (Coccus) and has a gram negative, the second type is rod-shaped bacteria (Bacilli) and has a gram negative; and there was only one type of nematode, namely Pratylenchus sp.