Determination of D-2-halopropionate dehalogenase activity from Pseudomonas putida strain AJ1/23 by ion chromatography

Abstract

A new assay for dehalogenase enzymes has been developed, based on the determination of halide ion concentration in assay samples by ion chromatography. The ion chromatograph is sensitive enough to allow routine measurement of chloride ions (C1-) down to 5 ng/sample (i.e. 50 μl of 0.1 ppm solution) and was used to determine the apparent Michaelis constant for D-2-chloropropionate of the D-2-halopropionate dehalogenase from Pseudomonas putida AJ1/23. © 1989.