Objective: To determine the effect of Matrigel at a low concentration on the growth of mouse embryos in culture. Design: Randomized case-control study of mouse embryos. Setting: An academic research environment. Animals: Mouse embryos. Intervention(s): Embryos were cultured in Quinn's or Celbio's human tubal fluid (HTF) enriched with 1.5% bovine serum albumin and 0.8% liquid Matrigel. Each HTF was compared with the same medium devoid of Matrigel. Afterward, Quinn's and Celbio's HTF, both containing Matrigel, were compared directly. Embryos were cultured in four-well dishes, and their morphology and viability were assessed at 96 hours. Main Outcome Measure(s): Level of interleukin-1α in media collected at the end of culture. Result(s): In both types of HTF, the presence of Matrigel allowed a larger number of embryos to reach the blastocyst stage and to hatch; blastocyst morphology also was improved. These positive effects were enhanced in Quinn's HTF: embryos cultured in its Matrigel-enriched version secreted a higher level of interleukin-1α than those in Celbio's HTF plus Matrigel and also showed a better morphology. Conclusion(s): In the mouse embryo model, Matrigel improves culture conditions in terms of both embryo viability and morphology, and these effects are enhanced in Quinn's HTF.
Lazzaroni, L., Fusi, F. M., Doldi, N., & Ferrari, A. (1999). The use of Matrigel at low concentration enhances in vitro blastocyst formation and hatching in a mouse embryo model. Fertility and Sterility, 71(6), 1133–1137. https://doi.org/10.1016/S0015-0282(99)00149-1