Microchip device with dry-stored reagents for Loop mediated isothermal amplification

Abstract

Storage of key reagents for amplification in a microfluidic device is decisive for point-of-care diagnostics. A simple approach of drying the reagents without special stabilizers by dehydration in a glass microchip is described. For loop mediated isothermal amplification of nucleic acids, a microchip device with two reactors was approbated. It was shown that the microchip device is suitable for detecting nucleic acids template of the Sacbrood virus. The experimental results obtained in microchip reactors with dry-stored primers were comparable to the results with non-dried primers. The shelf life of dehydrated primers without declined its reactivity turned out less than three months.