COMPARATIVE IN-VITRO ANTIOXIDANT SCREENING OF METHANOLIC EXTRACT OF COSTUS PICTUS & ITS SILVER NANOPARTICLES

Abstract

To compare in-vitro antioxidant activity of Methanolic extract of Costus pictus (MECP) and its Silver nanoparticles (MECPAgNPs) by various methods. Preliminary phytochemical screening of MECP was done by standard procedure. Synthesis of silver nanoparticles from MECP was done. In-vitro anti-oxidant activity of the MECP & MECPAgNPs were studied by DPPH assay, H2O2 scavenging activity, Phosphomolybdenum Method, FRAP and reducing power assay. The total Phenolic content, Flavonoid content & Vitamin C were estimated by using Gallic acid, Quercetin and standard Ascorbic acid calibration curve respectively. Preliminary phytochemical screening showed the presence of carbohydrates, triterpenoids, proteins, alkaloids, tannins, saponins, flavonoids, sterols and volatile oil. In-vitro antioxidant methods were resulted, the extract and the nanoparticles showed a dose dependent reducing ability. The nanoparticles at the same concentration offered much better activity than the extract alone. Phenolic content, Flavonoid content and Vitamin C amount of the MECPAgNPs was higher than MECP. These determination and quantification gives the information about the amount of secondary metabolites present in the MECPAgNPs was higher than the MECP which is responsible for the therapeutic or pharmacological activity of the plant. The MECPAgNPs showed very potent anti oxidant activity as compared to MECP.