Interleukin-8 in the pathogenesis of primary central nervous system lymphoma in association with HIV infection

Abstract

The pathogenesis of acquired immunodeficiency syndrome-associated primary central nervous system lymphoma (AIDS-associated PCNSL) remains unclear. However, cell adhesion molecules have been reported to be strongly associated with PCNSL. In this study, we established Epstein-Barr virus (EBV)- transformed lymphoblastoid cell lines (LCLs) from HIV-positive patients (LCLHIV) and normal individuals (LCLN). The expression of CD18 antigen by LCLHIV was stronger than that by LCLN. We performed a cell adhesion assay using ISO-HAS, which is the human hemangiosarcoma cell line and expresses intercellular adhesion molecule 1 (CD54). The binding rates of LCLHIV and ISO-HAS without stimulation were higher than those of LCLN. Further, we demonstrated that azidothymidine or simvastatin inhibited the binding rates of LCLHIV and ISO-HAS more significantly than those of LCLN. Further, the levels of interleukin (IL)-8, a CD18 inducer, were higher in LCLHIV than in LCLN. We conclude that interaction between IL-8 and CD18 may be critical to AIDS-related PCNSL. © W.S. Maney & Son Ltd 2012.