Dose-effect relationship on anti-inflammatory activity on LPS induced RAW 264.7 cells and antioxidant activity of rutin in vitro

Abstract

Rutin, as a common flavonol glycoside, exists abundantly in many plants and exhibits various nutritional and pharmacological function. The current study focused on the dose-effect relationship of rutin on the anti-inflammatory and antioxidant activities in vitro against LPS-stimulated RAW 264.7 cells and free radicals or ferric-ion. This study evaluated the anti-inflammatory activity by observation cell morphological and determination of the content of NO, TNF-α, IL-1β, and IL-6, and the phagocytic activity in LPS induced RAW 264.7 cells; and revealed the antioxidant activities via assay of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power. The study indicated that 5, 10, 20, 50 and 100 µM of rutin all exhibited a better anti-inflammatory activity, especially 50 and 100 µM. Meanwhile, it exerted stronger antioxidant activities than BHT, and equivalent to VC at the same concentration. Combined, the results suggested that rutin will be a potential and important adjuvant in the treatment of inflammatory diseases and oxidative stress.