Optimization of Lipase Production from Bacillus thuringiensis (TS11BP), Achromobacter xylosoxidans J2 (TS2MCN)–Isolated from Soil sediments near oilseed farm

Abstract

Aim: The aim of the present study is to estimate the optimum growth conditions of bacterial isolates TS11BP and TS2MCN having lipolytic activity. Materials and methods: Oil degrading bacteria were isolated from soil sample using serial dilution and pour plate method. Bacteria were further identified by morphological and biochemical tests and subjected to lipase production in 250 ml Erlenmeyer flask using Rhodamine olive oil medium and Tween 80 medium for 48h of fermentation period at 37 0 C with agitation speed of 150rpm in rotator shaker. Results: Different bacterial strains were isolated and screened for lipase production in submerged fermentation process. Among these two bacterial strains gave positive test for lipase production. The two strains were further characterized by biochemical and morphological tests and identified as Bacillus thuringiensis (TS11BP) and Achromobacter xylosoxidans (TS2MCN). Supplementation of various bacterial growth components such as Carbon, Nitrogen, Vitamin and Amino acids increased enzyme production. The optimum pH and temperature for the lipase activity of crude enzyme was 8 and 45 0 C for both the bacterial isolates. Maximum lipase production was recorded during 96hr of incubation period and 14% of inoculums level.