Influence of Organic Supplements on Growth and Development of In Vitro Shoots of Bulbophyllum dhaninivatii Seidenf

Abstract

In Vitro shoot culture of Bulbophyllum dhaninivatii Seidenf. was conducted on semi-solid Vacin and Went (1949) medium supplemented with coconut water (50 100 and 150 ml/L), potato extract (25 and 50 g/L) and banana homogenate (25 and 50 g/L) for 12 weeks. The results found that the highest shoot number (6.92 shoots) could observe on the medium supplemented with 150 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate while the highest leaf and root number could obtain when cultured on the medium supplemented with 100 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate.