Development of a multiplex polymerase chain reaction method for cps typing of Actinobacillus pleuropneumoniae serovars 1, 2, 5, 7, and 15

Abstract

We developed a multiplex polymerase chain reaction (PCR) method using primers specific to the capsular polysaccharide biosynthesis genes (cps) to type Actinobacillus pleuropneumoniae serovars 1, 2, 5, 7, and 15. This multiplex PCR method may be useful for the typing of serovar 15, which is recently becoming more prevalent, as well as the most prevalent serovars 1, 2, 5, and 7 in Japan.