IN-VITRO FREE RADICAL SCAVENGING POTENTIAL OF FLAVONOIDS WITH SYNERGISTIC EFFECT OF THEIR COMBINATION

Abstract

The present study was carried out to evaluate the possible synergistic interactions on antioxidant efficacy of some selected flavonoids in the present study, synergistic antioxidant effects of two flavonoids, rutin and quercetin, have been investigated by. DPPH, NO, free radical scavenging assays. Biological free radicals are highly unstable molecules that react with various organic substrates such as lipids, proteins, DNA causing cellular injury. At high concentrations, they generate oxidative stress, a damaging process that can damage all cell structures. At present, the research is focused on the use of antioxidants in preventing many diseases caused by the free radicals. The study was designed to evaluate in vitro antioxidant potential of quercetin, rutin, individually and synergistic antioxidant effects by using various in-vitro antioxidant assays i.e. DPPH, NO, assays.  The scavenging effect of quercetin, rutin and standard on the DPPH radical was 83±2.71 %, 79±1.51% and 86±1.04 % at 60μg/ml. On NO Quercetin, rutin and standard exhibited 83±0.09 %, 76±0.09 % and 85±0.22%. The scavenging activity increased in a dose dependent manner. The results indicate drugs shows significant free radical scavenging and their combination demonstrated considerable synergistic effect as compared to standard. Keywords: Quercetin, Rutin, Free radical scavenging, Antioxidant