Chitosan-based coatings to prevent the decay of Populus spp. Wood caused by trametes versicolor

Abstract

Chitosan and chitosan oligomers are receiving increasing attention due to their antimicrobial properties. In the present study, they were assayed as a preventive treatment against white-rot decay of Populus wood (very important in economic and environmental terms), caused by Trametes versicolor fungus. Their capacity to incorporate different chemical species into the polymer structure with a view to improving their anti-fungal activity was also assessed by mixing oligo-chitosan with propolis and silver nanoparticles. The minimum inhibitory concentration of medium-molecular weight chitosan (MMWC), chitosan oligomers (CO), propolis (P), nanosilver (nAg), and their binary and ternary composites against T. versicolor was determined in vitro. Although all products exhibited anti-fungal properties, composites showed an enhanced effect as compared to the individual products: 100% mycelial growth inhibition was attained for concentrations of 2.0 and 0.2 mg·mL-1 for the CO-P binary mixture, respectively; and 2 μg·mL-1 for nAg in the ternary mixture. Subsequently, MMWC, CO, CO-P and CO-P-nAg composites were tested on poplar wood blocks as surface protectors. Wood decay caused by the fungus was monitored by microscopy and vibrational spectroscopy, evidencing the limitations of the CO-based coatings in comparison with MMWC, which has a higher viscosity and better adhesion properties. The usage of MMWC holds promise for poplar wood protection, with potential industrial applications.