Species-specific detection of Erwinia pyrifoliae by PCR assay using enterobacterial repetivive intergenic consensus (ERIC) primers

Abstract

We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens. © The Korean Society of Plant Pathology.