Comparison of volatile profile and antioxidant activity of Piper divaricatum G. Meyer (Piperaceae) using cuttings and cell tissue

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Abstract

In this study, P. divaricatum (Piperaceae) plants were subcultured by cuttings and cell tissue in order to carry out a comparison of the volatile profile, phenolic compounds, and antioxidant activity. Propagation by cuttings used vermiculite substrate and Murashige-Skoog (MS) medium in the absence of growth regulators and rooting started at 15 days of growth providing a higher number of plants after 90 days. In vitro propagation was performed using shoot apices as explants in MS media supplemented with 0.5 mg mL-1 BA (6-benzyladenine). Volatile profiles analyzed by gas chromatography-mass spectrometry (GC-MS) showed as main compounds methyl eugenol, E-β-ocimene, and β-elemene in the in vivo and in vitro cultures. Phenolic contents determined by the Folin-Ciocalteu method had no significant difference at the end of 90 days of growth displaying a good linear correlation with antioxidant activity and phenylpropanoids amounts (r > 0.7). However, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging was higher for in vitro plants grown in comparison to in vivo plants cultured.

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Corpes, R., Monteiro, L., Andrade, E. H., Maia, J. G., Menezes, I., Setzer, W. N., … da Silva, J. K. R. (2019). Comparison of volatile profile and antioxidant activity of Piper divaricatum G. Meyer (Piperaceae) using cuttings and cell tissue. Journal of the Brazilian Chemical Society, 30(11), 2291–2298. https://doi.org/10.21577/0103-5053.20190128

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