Possibility of classification in some species of Lilium by PCR-RFLP of ribulose-1, 5-bisphosphate carboxylase large subunit (rbcL) gene and ribosomal RNA gene

Abstract

PCR-RFLP analysis was applied to classify nine Lilium species, L. japonicum, L. rubellum, L. nobilissimum, L. speciosum, L. auratum, L. auratum var. platyphyllum. L. henryi, L. concolor var, pulchellum and L. leichtlinii var. maximouiczii, and one interspecific hybrid, L. x formolongi. When PCR products amplified in specific regions of ribulose-1, 5-bisphosphate carboxylase large subunit (rbcL) gene in chloroplast and nuclear ribosomal RNA (rRNA) gene were digested by 20 restriction enzymes, some polymorphisms were observed The examined plants were classified into six groups based on the combined results in rbcL and rRNA gene. The tested species m Archelirion and Sinomartagon were classified into different groups. Furthermore six species in Archelirion were classified into three groups. Thus our study suggested that PCR-RFLP analysis could be used for classification of lilies.