The Bacterial SMC Complex Displays Two Distinct Modes of Interaction with the Chromosome

Citations of this article
Mendeley users who have this article in their library.


The bacterial SMC (structural maintenance of chromosomes) complex binds nonspecifically to DNA invitro and forms two discrete subcellular centers invivo, one in each cell half. How this distribution is maintained is unclear. We show by time-lapse imaging of single molecules that the localization is achieved through limited, yet rapid movement of the SMC subunits through the nucleoid. Accessory ScpAB subunits mediate the arrest of 20% of SMC molecules at the center of a cell half and do not move together with the 80% mobile SMC molecules. Only free SMC, but not the preformed SMC/ScpAB complex, was able to bind to DNA invitro, revealing distinct functions of SMC fractions. Thus, whereas SMC alone dynamically interacts with many sites on the chromosome, it forms static assemblies together with ScpAB complex partners. Our findings reveal two distinct modes of interaction of SMC with the chromosome and indicate that limited diffusion within a confined space and transient arrest may be a general mechanism for positioning proteins within a chromosome and within a noncompartmentalized cell. © 2013 The Authors.




KleineBorgmann, L. A. K., Ries, J., Ewers, H., Ulbrich, M. H., & Graumann, P. L. (2013). The Bacterial SMC Complex Displays Two Distinct Modes of Interaction with the Chromosome. Cell Reports, 3(5), 1483–1492.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free