We investigated the role of the integrins αvβ3 and αvβ5 in mediating vitronectin adhesion of three phenotypically distinct cell types. M21 human melanoma cells and H2981 lung carcinoma cells use both αv-containing integrins in adhering to vitronectin while UCLA-P3 lung carcinoma cells adhere exclusively with αvβ5. Specifically, monoclonal antibodies directed to functional epitopes on both receptors were required to block adhesion of M21 or H2981 cells while adhesion of UCLA-P3 cells to vitronectin could be blocked with a monoclonal antibody to αvβ5. Although both receptors are involved in M21 and H2981 cell adhesion to vitronectin, only αvβ3 can be detected in focal contacts, colocalizing with vinculin, talin, and the ends of actin filaments, while αvβ5 shows a distinct, nonfocal contact, distribution on the cell surface. These results provide the first evidence that two homologous integrins that recognize the same ligand distribute differentially on the cell surface.
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CITATION STYLE
Wayner, E. A., Orlando, R. A., & Cheresh, D. A. (1991). Integrins αvβ3 and αvβ5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. Journal of Cell Biology, 113(4), 919–929.