Microdetermination of Phosphorus

Abstract

The ascorbic acid method of Ammon and Hinsberg, modified by Lowry and associates, has been applied to the determination of phosphorus in whole blood, plasma, serum, and urine. A sensitivity about eight times that of the aminonaphtholsulfonic acid method permits the use of much smaller samples for measurement in conventional cells (as little as 0.15 γ of phosphorus can be determined in ordinary 3-ml. cuvettes.) A comparison with an accepted procedure on a number of samples showed that the ascorbic acid method gave essentially the same results. © 1956, American Chemical Society. All rights reserved.