Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA

Abstract

Products of mitochondrial protein synthesis in yeast have been labeled in vivo with 35SO42-. More than 20 polypeptide species fulfilling the criteria of mitochondrial translation products have been detected by analysis on sodium dodecyl sulfate exponential polyacrylamide slab gels. A comparison of mitochondrial translation products in two wild type strains has revealed variant forms of some polypeptide species which show genetic behavior consistent with the location of their structural genes on mtDNA. The results demonstrate the feasibility of performing genetic analysis on putative gene products of mtDNA in wild type yeast by direct examination of the segregation and recombination behavior of specific polypeptide species.