Development of HPTLC Method and Its Validation for the Estimation of Curcuminoids from Polyherbal Mouth Ulcer Gel Formulation

Abstract

A rapid, simple, selective and precise high performance thin layer chromatographic method has been developed for the determination of curcumin in mouth ulcer poly herbal formulation. The separation was performed on TLC aluminium plates Precoated with silica gel G60 F254. Good separation was achieved in the mobile phase of Chloroform: methanol: Glacial acetic acid (7.5: 2.0: 0.5 v/v/v) at Rf = 0.18, 0.31, 0.56 for bisdemethoxy curcumin, demethoxy curcumin and curcumin respectively. The method was validated successfully by checking precision, repeatability and accuracy. The present method can simplify the analysis of Curcuminoids in poly herbal formulations for their routine quality analysis.