Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum

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Abstract

The practical advantages of sampling and storing blood on filter paper for analyses of human and pathogen genes highlight the need for reliable, sensitive, and cost-effective DNA extraction methods. We describe a new Tris-EDTA (TE) buffer-based method for extraction of DNA from blood dried on filter paper. The method was evaluated against the commonly used methanol and Chelex® methods, regarding polymerase chain reaction detection of Plasmodium falciparum parasites from samples stored for 1-2 years. The sensitivity of detection was dependent on the parasite density and type of filter paper. For 3MM® Whatman filter paper, the sensitivity was 100%, 73%, and 93% for the TE, methanol, and Chelex® methods, respectively. For the longer stored 903® Schleicher & Schuell filter paper, the sensitivity was 93%, 73%, and 0%, respectively. This rapid, simple, and inexpensive extraction method generated superior results from archived specimens compared with the two standard methods and may represent a useful tool in molecular epidemiologic studies. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.

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APA

Bereczky, S., Mårtensson, A., Gil, J. P., & Färnert, A. (2005). Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum. American Journal of Tropical Medicine and Hygiene, 72(3), 249–251. https://doi.org/10.4269/ajtmh.2005.72.249

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