Light Scattering to Quantify Protein–Protein Interactions at High Protein Concentrations

Abstract

Static and dynamic (laser) light scattering (SLS and DLS, respectively) can be used to measure the so-called weak or colloidal protein–protein interactions in solution from low to high protein concentrations (c2). This chapter describes a methodology to measure protein–protein self-interactions using SLS and DLS, with illustrative examples for monoclonal antibody solutions from low to high protein concentrations (c2 ~ 1–102 g/L).