Distribution of α(1B)-adrenergic receptor mRNA expression along rat nephron segments

Abstract

Although several α-adrenergic receptor genes are expressed i the rat kidney, little information is available on their expression in the renal nephron segments. We investigated the distribution of α(1B)-adrenergic receptor mRNA in rat nephron segments using reverse transcription and polymerase chain reaction (RT-PCR). The nephron segments of six- to eight- week-old male Sprague-Dawley rats were microdissected. Total RNA was prepared by the acid-guanidinium-phenol-chloroform method and used in the following RT-PCR assay. The PCR products were size-fractionated with electrophoresis, visualized with ethidium bromide staining and confirmed by Southern blot analysis. Because the PCR primers spanned an intron, the amplification product of the predicted size was considered to be from α(1B)-adrenergic receptor cDNA and not from genomic DNA. The PCR products were detected in glomerulus (Glm), proximal convoluted and straight tubules (PCT, PST) and cortical and medullary thick ascending limbs of Henle (CTAL, MTAL). No signals were detected in cortical or medullary collecting ducts (CCD, MCD). Large signals were detected in the PCT, and PST< while small signals were found in the Glm. CTAL and MTAL. The α(1B)-adrenergic receptor mRNA was detected for the first time in rat Glm, PCT, PST and TAL using RT-PCR, α(1B)AR mRNA seems to be expressed in the specific sites along the nephron and may play significant roles in renal functions, although the specific physiological effects of the renal α(1B)-adrenergic receptor are unknown.