Characterization, diversity, and effectiveness phosphate solubilizing bacteria from the soil and rhizosphere on the growth of Glycine max L. in greenhouse

Abstract

The research was conducted to know the diversity, characterization, and effectiveness of phosphate solubilizing bacteria from the soil and rhizosphere on the growth of Glycine max L. in a greenhouse. The study aims to know the population, get the pure culture of phosphate solubilizing bacteria, and obtain isolates potential of the soybean growth. Isolation was done in medium Pycosvkaya. The growth characteristic of pure culture was observed using Pikovskaya medium; the clear zone surrounding the colonies indicates that the phosphate in the medium was hydrolyzed. The experiment aimed to assess the potency of the Phosphate Solubilizing Bacteria to increase the growth of Glycine max L, using sand media sterilized. The strain used selected; there were ten isolates used in this research. Parameters of the investigation were the dry weight of canopy, roots, root nodules, total plants, number of nodules, and "Symbiotic Capacity."The highest population was found from the sample soil from the rhizosphere with Sechium edule plant. Forty-five gave of pure culture, wholly able to hydrolyzed phosphate. This study showed that the strain number of 9 BTLP(2) (isolate from Zea mays plant) had given the best results on the growth of Glycine max L.