Discovery pipeline for epigenetically deregulated miRNAs in cancer: Integration of primary miRNA transcription

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Abstract

Background: Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer.Results: Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells.Conclusions: We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression. © 2011 Hulf et al; licensee BioMed Central Ltd.

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Hulf, T., Sibbritt, T., Wiklund, E. D., Bert, S., Strbenac, D., Statham, A. L., … Clark, S. J. (2011). Discovery pipeline for epigenetically deregulated miRNAs in cancer: Integration of primary miRNA transcription. BMC Genomics, 12. https://doi.org/10.1186/1471-2164-12-54

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