Compacting DNA during the interphase: Condensin maintains rDNA integrity

Abstract

During mitosis, condensin is responsible for folding chromatin fibers into highly compact chromosomes, ensuring the faithful segregation of replicated chromosomes into daughter cells after each cell division. Our laboratory has unexpectedly found that condensin is capable of condensing DNA during the interphase: upon nutrient starvation, condensin is loaded to the rDNA array leading to DNA condensation in this region. This subchromosomal DNA condensation appear to protect the integrity of the rDNA array. These observations provide the first microscopic evidence of DNA compaction by condensin outside mitosis. In addition, they show that condensin is highly regulated outside mitosis. ©2007 Landes Bioscience.