Photoel ectroanalytical Detection of adrenaline based on DNA and TiO2 nanoparticles sensitized with bis(ethylenedithio) tetrathiafulvalene exploiting led light

Abstract

In this study, a photoelectroanalytical sensor for determination of adrenaline based on deoxyribonucleic acid (DNA) and anatase titanium dioxide (TiO2) nanoparticles sensitized with bis(ethylenedithio)tetrathiafulvalene (BEDT-TTF) was developed, which we henceforward call BEDT-TTF/DNA/TiO2/ITO. The photoelectroanalytical sensor showed high photocurrent to adrenaline under visible light emitting diode (LED) light irradiation in comparison to each component of the composite material. Under optimized conditions, the BEDT-TTF/DNA/TiO2/ITO sensor shows a linear response range from 10 nmol L-1, up to 100 μmol L-1, with a sensitivity of 8.1 nA L μmol-1, limit of detection of 1 nmol L-1, for the detection of adrenaline. The photoelectrochemical sensor showed high photocurrent to adrenaline in comparison to photocurrent response to ascorbic acid and uric acid. The BEDT-TTF/DNA/TiO2/ITO photoelectrochemical sensor was successfully applied to urine samples, with recovery values between 96 and 106%.