Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β-catenin pathway

Abstract

Benzyl isothiocyanate (BiTc) has been reported to exhibit antitumor properties in various cancer types; however, the Underlying mechanisms of its action remain unclear. In the present study, the efficacy of BITC on murine mammary carcinoma cells was evaluated in vitro and in vivo, revealing a potential mechanism for its action. In vivo bioluminescence imaging indicated dynamic inhibition of murine mammary carcinoma cell growth and metastasis by BiTc. a terminal deoxynucleotidyl transferase-mediated duTP nick end labeling assay demonstrated that BiTc also induced apoptosis. BiTc further exhibited antitumorigenic activity in 4T1-luc cells in vitro via the inhibition of cell proliferation, induction of apoptosis and cell cycle arrest, and inhibition of cell migration and invasion. Furthermore, the activity of key molecules of the adenomatous polyposis coli (aPc)/β-catenin complex was altered following treatment with BiTc, which suggested a potential role for the aPc/β-catenin complex in the BiTc-mediated induction of apoptosis and inhibition of metastasis in murine mammary carcinoma. BiTc upregulated the activity of glycogen synthase kinase-3β and aPc proteins, whereas it downregulated β-catenin expression. The inhibition of metastasis was accompanied with the downregulation of vimentin and upregulation of e-cadherin. conversely, BiTc did not exhibit toxicity or side effects in the normal mammary epithelial cell line McF-10a. The present study indicated that BiTc exhibited anticancer properties due to the induction of breast cancer cell apoptosis and inhibition of breast cancer cell metastasis mediated by the Wnt/β-catenin signaling pathway.