Blue light inhibits slime mold differentiation at the mRNA level.

8Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The influence of blue light on protein synthesis in spherulating Physarum polycephalum microplasmodia was studied using two-dimensional protein separation techniques. The starvation-induced plasmodium-spherule transition proceeds in the dark and is accompanied by the synthesis of 20 major differentiation-specific proteins as revealed by in vivo labelling with [35S]methionine. Three of these proteins are identical with cell wall components with respect to their mol. wts. (35 K, 34 K and 14 K) and isoelectric points. Spherulation is also accompanied by the appearance of 26 prominent differentiation-specific mRNA species translatable in the rabbit reticulocyte cell-free system. Six of the proteins synthesized in vitro co-migrate on two-dimensional gels with proteins labelled in vivo, two of them being cell wall components. Blue light, which inhibits spherulation completely, inhibits also the synthesis of spherule proteins and of spherule-specific mRNA activity. Only three protein components are induced by blue light, indicating that illumination does not induce a novel differentiated plasmodial state.

Cite

CITATION STYLE

APA

Putzer, H., Werenskiold, K., Verfuerth, C., & Schreckenbach, T. (1983). Blue light inhibits slime mold differentiation at the mRNA level. The EMBO Journal, 2(2), 261–267. https://doi.org/10.1002/j.1460-2075.1983.tb01415.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free