α‐D‐Galactosidase (EC 3.2.1.22) from Bifidobacterium longum

Abstract

α‐Galactosidase activity in Bifidobacterium longum was maximal in a range of 40–45°C and at pH of 5.8. At temperatures above 60°C the enzyme was completely inactivated, but it maintained a 100% activity during storage at low temperatures (4, ‐ 30, ‐ 70°C) for 3 months. The addition of Hg2+ to the reaction buffer produced a strong inhibitory effect while Mn2+ exerted a slight positive effect upon activity. The addition of EDTA, inhibitors and other metal ions had no effect on the enzyme α‐galactosidase. Copyright © 1994, Wiley Blackwell. All rights reserved