The force-generation process in active muscle is strain sensitive and endothermic: A temperature-perturbation study

Abstract

In experiments on active muscle, we examined the tension decline and its temperature sensitivity at the onset of ramp shortening and at a range of velocities. A segment (∼1.5 mm long) of a skinned muscle fibre isolated from rabbit psoas muscle was held isometrically (sarcomere length ∼2.5 μm) at 8-9°C, maximally Ca2+-Activated and a ramp shortening applied. The tension decline with a ramp shortening showed an early decrease of slope (the P1 transition) followed by a slower decrease in slope (the P2 transition) to the steady (isotonic) force. The tension level at the initial P1 transition and the time to that transition decreased as the velocity was increased; the length change to this transition increased with shortening velocity to a steady value of ∼8 nm half-sarcomere-1. A small, rapid, temperature jump (T-jump) (3-4°C, <0.2 ms) applied coincident with the onset of ramp shortening showed force enhancement by T-jump and changed the tension decline markedly. Analyses showed that the rate of Tjump-induced force rise increased linearly with increase of shortening velocity. These results provide crucial evidence that the strainsensitive cross-bridge force generation, or a step closely coupled to it, is endothermic.