Antimicrobial Action of Peracetic Acid (2)

Abstract

Peracetic acid acted on purine and pyrimidine bases, and these nucleosides. This action was markedly affected by pH in reaction media. Peracetic acid also acted on deoxyribo-nucleic acid (DNA). It has been revealed through the base analysis of DNA that one of the actions of peracetic acid on DNA is the action on the base constituents. Their decomposition by peracetic acid was stronger under acidic condition than under neutral or alkaline condition. In addition, the decrease of the viscosity of DNA solution was observed. It was suggested that the decrease may be due to degradation of DNA molecule such as the hydrolysis of internucleotidic linkages, etc. (Received December 17, 1973) ‰ß •| Ž_ ‚Ì ‹-‚¢ ŽE‹Û-Í,•L ‚¢ •R‹Û ƒXƒy ƒN ƒgƒ‹ ‚É‚Â ‚¢ ‚Ä‚Í ‚±‚ê ‚Ü ‚Å‚É ‚à'½ ‚-‚Ì•ñ ••1)•`4)‚ª ‚ ‚邪,‰ß •| Ž_ ‚ÌŽE ‹Û ‹@ •\ ‚̉ð-¾‚ð-Ú"I ‚AE ‚µ‚½OE¤ ‹ † ‚Í ‚Ù ‚AE‚ñ ‚ÇOE© ‚¢ ‚¾ ‚¹ ‚È ‚© ‚Á ‚½.'˜ ŽÒ ‚ç‚Í 'O •ñ5)‚É ‚¨‚¢ ‚Ä‰ß •|Ž_ ‚Ì ŽE ‹Û‹@ •\ ‚ð OEŸ "¢ ‚µ,‰ß •| Ž_ ‚Ì ŽE ‹Û •ì-p ‚Í‰ß •|Ž_ ‚ª "÷• ¶ •¨ ‚Ì "Á'è ‚Ì•×-E •\ '¢ ‚â • ¶ Šˆ‹@Šˆ‹@ "\ ‚É "ÁˆÙ "I ‚É•ì-p ‚· ‚éOE‹ ‰Ê ‚Å ‚Í ‚È ‚-,•×-E-OE ‚â •×-E Ž¿ ‚Ì •\ •¬ •¬ •ª ‚ ‚é ‚¢‚Í •×-EŽ¿ ‚̉Â-n •« •¬ •ª ‚È ‚Ç•s "Á 'è •¬ •ª ‚AE‚Ì •L ‚¢"½ ‰ž ‚É ‚ae ‚é ‚à‚Ì ‚Å ‚ ‚é ‚AE•" 'è ‚µ‚½.‚± ‚Ì•" 'è ‚ÉŠî ‚Ã ‚«•¡ ‰ñ ‚Í ‚AE ‚-‚É "÷ • ¶ •¨ ‚Ì• ¶ ˆç •ã •d-v ‚È"-‚« ‚ð-L ‚· ‚éŠj Ž_ ‚È ‚ç‚Ñ ‚ÉŠj Ž_ ‰-Šî ‚É 'Î ‚· ‚é‰ß •| Ž_ ‚Ì•ì-p ‚É ‚ ‚¢ ‚ÄOEŸ "¢ ‚µ,‹»-¡ ‚ ‚éOE‹ ‰Ê ‚ª "¾ ‚ç‚ê ‚½ ‚Ì‚Å •ñ•• ‚· ‚é. ŽÀ OE± •Þ-¿ Šj Ž_ ‰-Šî-Þ ˜a OEõ•ƒ-ò •H ‹AE Š" Ž® ‰ï ŽÐ ‚Ì"Á ‹‰ •i ‚ð-p ‚¢‚½. ‰ß •| Ž_ •Ð ŽR•H ‹AE Š" Ž® ‰ï ŽÐ ‚Ì•» •i(‰ß •| Ž_ ‚ÌŠÜ-Ê9%)‚ð-p ‚¢ ‚½. Šj Ž_(DNA) Že ƒE ƒV‚Ì ‹¹ 'B‚© ‚çSDS-@6)‚É •] ‚Á ‚Ä'² •» ‚µ‚½. " § •Í-OE ƒrƒX ƒL ƒ" ƒOŽÐ ‚Ì" § •Í-p ƒZ ƒ‹ ƒ••[ ƒY ƒ` ƒ…•[ ƒu‚ð-p ‚¢ ‚½. ŽÀ OE± ‚¨‚ae‚Ñ‚¨‚ae‚Ñ OE‹‰Ê 1. Šj Ž_ ‰-Šî ‚É'Î ‚· ‚é‰ß •| Ž_ ‚Ì•ì-p •Š 'è ‚ÌpH‚É '² •» ‚µ‚½0.1M‰ß •| Ž_10ml‚É10mg ‚̉-Šî(ƒk ƒN ƒOEƒI ƒV ƒh‚Ì•ê • ‡ ‚Í ŠÜ ‚Ü‚ê ‚é‰-Šî-Ê ‚ð10mg ‚É ‚È ‚é ‚ae ‚¤‚É Š· ŽZ ‚µ‚½)‚ð-n ‰ð OEã,37•‹ ‚Å48Žž ŠÔ"½ ‰ž ‚³ ‚¹ ‚½.ƒO ƒA ƒj ƒ"‚Ì •ê• ‡ ‚Í •… ‚É 'Î ‚· ‚é-n ‰ð •« ‚ª'á ‚¢ ‚½‚ß, ‚Ü‚¸0.1M•… Ž_ ‰» ƒi ƒgƒŠƒE ƒ€-n ‰t0.5ml‚É-n ‰ð OEã, •Š'è ‚ÌpH‚É '² •® ‚µ‚½0.1M‰ß •| Ž_-n ‰t9.5ml‚ð "Y ‰Á ‚µ‚Ä "¯-l ‚É "½ ‰ž ‚ð ŠJ Žn ‚µ‚½.0.1M•… Ž_ ‰» ƒi ƒg ƒŠƒEƒ€-n ‰t ‚É-n ‰ð ‚µ‚½ ‚½ ‚ß ‚ÌpH‚ÌˆÚ ‚ÌpH‚ÌˆÚ "® ‚Í ‰ß •| Ž_ ‚ÌŠÉ •Õ"\ ‚ª 'å ‚«‚¢ ‚½ ‚ß ‚Ù ‚AE‚ñ ‚Ç"F ‚ß ‚ç‚ê ‚È‚© ‚Á‚½.‚± ‚Ì "½ ‰ž ‰t ‚ð•… ‚Å200"{ ‚ÉŠó Žß ‚µ‚½ ‚Ì ‚¿,Še ‰-Šî ‚ÌŽ ‡ ŠO •" ‚Å ‚Ì •Å 'å ‹zŽû "g '·(ƒA ƒf ƒj ƒ"260nm,ƒO ƒA ƒj ƒ"250nm,ƒV ƒgƒV ƒ" 275nm,ƒ` ƒ~ ƒ"265nm,ƒE ƒ‰ƒV ƒ‹260nm)‚Å ‹z OEõ"x ‚Ì OE¸•OE¸•-‚ð 'ª'è ‚µ‚½.ƒu ƒ‰ ƒ" ƒN ‚AE ‚µ‚ĉ-Šî ‚ð ŠÜ ‚Ü‚È ‚¢ ‹ae ‚ð ‚AE ‚è,ŽŽ OE± ‹ae,'Î •AE ‹ae ‚ae ‚èOE¸‚‚èOE¸‚ ¶‚½.OE‹ ‰Ê ‚Ì •\Ž¦ ‚ÍŠÉ •Õ ‰t ‚AE *‹ã •B 'å Šw "_ Šw •":•Ÿ ‰ªŽs " •è