Kinetic spectrophotometric method for the estimation of Ofloxacin in pharmaceutical formulations

Abstract

The objective of the current study was to develop a direct, sensitive spectrophotometric method based on the oxidation of Ofloxacin using potassium permanganate in alkaline medium. The rate of change of absorbance was measured at 603 nm. The initial rate method and fixed time method (at 4 min) are utilised to construct calibration graphs for calculating the concentration of the drug. The results were validated through inter day and intraday precision assays according to the ICH guidelines and also through recovery studies. Statistical comparison of the proposed methods with that of reference method shows excellent agreement and indicates no significant difference in their accuracy and precision. © 2012, Sociedad Química de México.