Probing the molecular determinants of fluorinase specificity

Abstract

Molecular determinants of FlA1 fluorinase specificity were probed using 5′-chloro-5′-deoxyadenosine (5′-ClDA) analogs as substrates and FlA1 active site mutants. Modifications at F213 or A279 residues are beneficial towards these modified substrates, including 5′-chloro-5′-deoxy-2-ethynyladenosine, ClDEA (>10-fold activity improvement), and conferred novel activity towards substrates not readily accepted by wild-type FlA1.