Characterization of the meta-cleavage compound hydrolase gene involved in degradation of the lignin-related biphenyl structure by Sphingomonas paucimobilis SYK-6

Abstract

Sphingomonas paucimobilis SYK-6 has the ability to transform a lignin- related biphenyl compound, 2,2'-dihydroxy-3,3'-dimethoxy-5,5'- dicarboxybiphenyl (DDVA), to 5-carboxyvanillic acid (5CVA) via 2,2',3- trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (OH-DDVA). In the 4.9-kb HindIII fragment containing the OH-DDVA meta-cleavage dioxygenase gene (ligZ), we found a novel hydrolase gene (ligY) responsible for the conversion of the meta-cleavage compound of OH-DDVA to 5CVA. Incorporation of 18O from H218O into 5CVA indicated there was a hydrolytic conversion of the OH- DDVA meta-cleavage compound to 5CVA. LigY exhibited hydrolase activity only toward the meta-cleavage compound of OH-DDVA, suggesting its restricted substrate specificity.