discordia1 and alternative discordia1 function redundantly at the cortical division site to promote preprophase band formation and orient division planes in maize

Abstract

In plants, cell wall placement during cytokinesis is determined by the position of the preprophase band (PPB) and the subsequent expansion of the phragmoplast, which deposits the new cell wall, to the cortical division site delineated by the PPB. New cell walls are often incorrectly oriented during asymmetric cell divisions in the leaf epidermis of maize (Zea mays) discordia1 (dcd1) mutants, and this defect is associated with aberrant PPB formation in asymmetrically dividing cells. dcd1 was cloned and encodes a putative B'' regulatory subunit of the PP2A phosphatase complex highly similar to Arabidopsis thaliana FASS/TONNEAU2, which is required for PPB formation. We also identified alternative discordia1 (add1), a second gene in maize nearly identical to dcd1. While loss of add1 function does not produce a noticeable phenotype, knock down of both genes in add1(RNAi) dcd1(RNAi) plants prevents PPB formation and causes misorientation of symmetric and asymmetric cell divisions. Immunolocalization studies with an antibody that recognizes both DCD1 and ADD1 showed that these proteins colocalize with PPBs and remain at the cortical division site through metaphase. Our results indicate that DCD1 and ADD1 function in PPB formation, that this function is more critical in asymmetrically dividing cells than in symmetrically dividing cells, and that DCD1/ADD1 may have other roles in addition to promoting PPB formation at the cortical division site. © 2009 American Society of Plant Biologists.