Effect of urea—type citokinins on axillary shoots regeneration of impatiens walleriana l.

Abstract

To establish an efficient procedure for in vitro plant regeneration of Impatiens walleriana L., the effects of urea-type cytokinins, forchlorofenuron (CPPU) and thidiazuron (TDZ) on the axillary shoot induction are examined. The shoot meristem explants produced multiple axillary shoots when cultured on Murashige and Skoog's (MS) medium containing different concentration of CPPU and TDZ (0.01–1.0 μM). Explant activity varied significantly depending on the cytokinin type as well as concentration. We have found that the 0.1 μM CPPU induced the largest number of axillary shoots (4.89). This cytokinin was more effective for the induction and development of axillary shoots than TDZ in the same concentration. The separated shoots are rooted and elongated on MS medium without hormone. Rooted plantlets were successfully acclimatized. Shoot meristem explants and urea-type cytokinins could be useful for improvement of regeneration procedure and genetic engineering of this important ornamental species. © 2008 Taylor and Francis Group, LLC.