Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15

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Abstract

Hrs-2, via interactions with SNAP-25, plays a regulatory role on the exocytic machinery. We now show that Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and α-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. These data show that Hrs-2 regulates endocytosis, delineate a biochemical pathway (Hrs-2- Eps15-AP2) in which Hrs-2 functions, and suggest that Hrs-2 acts to provide communication between endo- and exocytic processes.

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Bean, A. J., Davanger, S., Chou, M. F., Gerhardt, B., Tsujimoto, S., & Chang, Y. C. (2000). Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15. Journal of Biological Chemistry, 275(20), 15271–15278. https://doi.org/10.1074/jbc.275.20.15271

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