Histomorphometric evaluation of the effects of local application of red clover oil (Trifolium pratense) on bone healing in rats

Abstract

Objective: Red clover oil (Trifolium pratense) that have isoflavones bunches which estrogen-like exercises and may establish an option in contrast to hormone substitution treatment. The present examination researched the impacts of Red clover oil on bone healing in rats by histomorphometrical study Methods: Intra bony defect was done in right femur of thirty six males healthy albino rats.Then these rats were randomly divided into three groups (12 rats for each)one control and 2 experimental group. For control, the bony defect left for normal healing, experimental (S)group the defect treated with hemostatic absorbable gelatin sponge,and for experimental (RS)group the bony defect treated with 0.2 ml red clover oil and covered by haemostatic absorbable gelatin sponge. Six rats from each group were sacrificed at 2 and 4 weeks intervals. Histolomorphometric analysis was performed on H&E bone section of all the studied groups which include counting of bone cells (osteoblasts, osteocytes and osteoclasts), trabecular number, trabecular area and bone marrow space area Results: Histomorphometrical results for bone cells revealed that combination group stimulated large number of osteoblasts and osteocytes than in sponge and control group. Number of new bone trabeculae, trabecular area and bone marrow space area showed high mean value in combination groups than others. Highly significant group difference was observed in all histomorphometric parameters in all duration. Conclusion:Red clover oil stimulated large number of osteoblasts and osteoclasts that indicate increase bone remodeling especially at 2 weeks interval when compared with sponge and control group.