Automated, quantitative cytopathic effect reduction assay for interferon

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Abstract

A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.

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Yeh, T. J., McBride, P. T., Overall, J. C., & Green, J. A. (1982). Automated, quantitative cytopathic effect reduction assay for interferon. Journal of Clinical Microbiology, 16(2), 413–415. https://doi.org/10.1128/jcm.16.2.413-415.1982

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