COMPARATIVE WOUND HEALING ACTIVITY OF PLANT ICHNOCARPUS FRUTESCENS (L.) W. T. AITON

Abstract

The present work objective are to investigate indigenous plants used in wound healing in India, we hereby reported our findings related to wound healing activities of plants Ichnocarpus frutescens (L.) W. T. Aiton in vivo model studies. The plant hydro-alcoholic (Ethanol 70:Water 30) extracts of the leaves, stems and roots of Ichnocarpus frutescens was identified and tested wound healing activity. The important secondary metabolites like Ursolic acid, Kaempferol, Trifolin, Mannitol, Flavon, Phenolic acid, Triterpene glycoside(1,4)- beta -D-glucopyranosyl-(1,3) alpha -amyrin, beta -Sitosterol, alkaloid and Flavonoid identified in extracts. Ichnocarpus frutescens roots hydro-alcoholic extract showed wound healing activity significantly in the excision wound model in rats on topical application. The animals were divided into five groups with six rats in each group. Topically applied Ichnocarpus frutescens leaves, stems and roots extracts ointment. Taking povidone iodine ointment as standard. The results showed that hydro-alcoholic extract of Ichnocarpus frutescens stems on topical application was reduced the scar area from 2.5+or-1.5 to 0.0+or-0.0 cm 2, hydro-alcoholic extract of Ichnocarpus frutescens leaves on topical application was reduced the scar area from 2.5+or-1.5 to 0.2+or-0.0 cm 2, hydro-alcoholic extract of Ichnocarpus frutescens roots on topical application was reduced the scar area from 2.5+or-1.5 to 0.7+or-0.4 cm 2, control on topical application was reduced the scar area from 2.5+or-1.5 to 1.2+or-1.0 cm 2 and standard povidon iodine ointment on topical application was reduced the scar area from 2.5+or-1.5 to 0.5+or-0.02 cm 2 respectively. Significant increase in tensile strength were also observed and compared to the control and povidon iodine. The above result revealed that the hydro-alcoholic stems extract has remarkable wound healing potency and appear to justify the traditional use of Ichnocarpus frutescens in wound healing in India and offer a scientific support to the treatment of traditional healers.