Background: Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family Togaviridae. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia. Findings. In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect and virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis. Conclusion: INA completely inactivated CHIKV and can further be explored for developing an inactivated-CHIKV vaccine. © 2012 Sharma et al.; licensee BioMed Central Ltd.
CITATION STYLE
Sharma, A., Gupta, P., & Maheshwari, R. K. (2012). Inactivation of Chikungunya virus by 1,5 iodonapthyl azide. Virology Journal, 9. https://doi.org/10.1186/1743-422X-9-301
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