Effect of Exposure to E-Cigarette Vapor and Cigarette Smoke on Seminiferous Tubules Diameter and Spermatozoa Quality of Mice (Mus musculus)

Abstract

This study aims to compare the quality of spermatozoa (motility and viability) and the diameter ofseminiferous tubules of mice (Mus musculus) by exposing to cigarette smoke and electronic cigarette vapor.This study used 28 male mice divided into 7 groups. Treatment group C was exposed to air, group A1given 1 cigarette/day, group A2 given 2 cigarettes/day, group A3 given 3 cigarettes/day, group B1 given0.7 ml/day, group B2 given 1,3 ml/day, group B3 given 2 ml/day. The treatments were carried out for 36days. The result of this study showed significant difference (p<0.05) in spermatozoa motility percentagebetween C, A1, A2, A3, B3, but in B1 showed no significant difference (p>0,05) with B2. Spermatozoaviability percentage showed significant difference (p<0.05) between control and all treatment groups.Diameter of seminiferous tubules showed significant difference (p<0,05) between C, A1, A2, A3, B1, B3, butin B2 showed no significant difference (p>0.05) with A1 and A2. In conclusion, this study showed that bothcigarette smoke and e-cigarette vapor exposure can decrease the quality of spermatozoa and diameter ofseminiferous tubules. E-cigarette vapor exposure groups showed better quality of spermatozoa and greaterdiameter of seminiferous tubules compared to cigarette smoke exposure groups.