Mycothiol/mycoredoxin 1-dependent reduction of the peroxiredoxin AhpE from mycobacterium tuberculosis

Abstract

Background: Mycothiol, the major low-molecular weight thiol of Mycobacterium tuberculosis, is important for peroxide detoxification and virulence. Results: Mycothiol, together with mycoredoxin-1, a glutaredoxin-like protein, reduces the one-cysteine peroxiredoxin AhpE from the bacterium. Conclusion: Mycobacterium tuberculosis AhpE is a mycothiol/mycoredoxin-1- dependent peroxidase. Significance: Our results provide the first molecular link between a thiol-dependent peroxidase and the mycothiol/mycoredoxin- 1 pathway in Mycobacteria. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.