Binding properties of biosynthesized gold nanoparticles with calf-thymus DNA in vitro

Abstract

The investigation of drug-DNA interaction is important for understanding the molecular mechanism of drug action. Interaction of ct-DNA with Alternanthera sessilis, Acorus calamus, Rhaphidophora aurea, Portulaca oleracea and Sterculia foetida mediated AuNPs was monitored by recording the changes in absorption band before and after interaction of ct-DNA and AuNPs by UV-Visible spectroscopy. The interaction of the complex with calf thymus DNA (ct-DNA) showed blue shift and hyperchromism in the UV-Vis spectra indicating groove binding for SF, PAK, MP and AC-AuNPs. The morphology of the DNA-nanoparticle complexes seems to be dependent on the nanoparticles concentration and is significantly different for each plant-mediated nanoparticles.