Capillary electrophoresis of nucleobases and nucleosides detected by TOF/MS

Abstract

The TOF/MS detection of nucleobases and nucleosides in capillary electrophoresis using a silica capillary combined with a stainless-steel electrospray ionization interface was studied under acidic conditions. The results showed that the nucleobases were migrated and detected as protonated species, while the nucleosides were detected by TOF/MS as the original nucleosides and the nucleobases fragment formed by collision-induced dissociation, after the migration of nucleosides as protonated species.