Callus induction studies in aquilegia nivalis Flac Ex jackson: An endangered medicinal plant of kashmir himalaya: A perspective

Abstract

Conventional propagation methods take a long time for multiplication because of low rate of growth set or poor germination of the seeds but tissue culture has an important role to play in the manipulation of plants for improved agronomic performance using different concentrations and combinations of plant growth regulator. Murashige and Skoog (MS) media with different combinations and concentrations of growth promoters i.e., auxin (Indole Acetic Acid (IAA), Indole Butyric Acid (IBA)), +synthetic auxin 2, 4-D (2, 4 dichlorophenoxy acetic acid), cytokinin (Benzyl Amino Purine (BAP), +synthetic cytokinin (kinetin (KN) and gibberellin (Gibberalic Acid (GA3). The efficient development of callus type was observed in the MS media supplemented with NAA (2 mg L-1) +kin (2 mg L-1). Three different types of explants viz., leaf (Petiole, middle and tip regions), shoot tips, root and petioles were cultured on MS medium containing 2,4 D, NAA, kinetin and their combinations. Callus was induced from petiole of the leaves of the Aquilegia nivalis. Highest and earliest callus formation (29 days) were observed on MS medium containing NAA (1.5 mg L-1) +kinetin (1 mg L-1). Callus proliferation was observed on 2, 4D (2 mg L-1) +kinetin (2 mg L-1) containing medium. Callus induction was not influenced by the season of the explant condition.