The effect of diosmin on pancreatic injury induced by hepatic ischemia reperfusion in rats

Abstract

Background: Hepatic ischemia-reperfusion causes histological injury to the pancreatic cells during transplantation,trauma and emergency surgery. We investigated the effects of diosmin, a phlebotrophic drug with antioxidant and antiinflammatory effects, on pancreatic injury in the experimental liver ischemia-reperfusion model. Materials and methods: Forty rats were divided into the four groups: sham (Group 1), control (Group 2), peroperative diosmin (50 mg/kg) treatment (Group 3) and preoperative 10-day diosmin (50 mg/kg) treatment group (Group 4). Ischemia-reperfusion model was carried out by clamping the hepatic pedicle for 60 min and then reperfusing the liver for 90 min. At the end of the procedures, blood and pancreas tissue samples were obtained for biochemical and histopathological assessment. Results: According to the results of liver function tests, amylase and the plasma and pancreatic tissue oxidative stress parameters, there was asignificant difference between the control and other groups. Histopathologically; the specimens of the Group 2 showed specific morphological abnormalities. The groups 3 and 4 showed the pancreas histomorphology similar to the sham group. Pathological scores were significantly different between the Group 2 and other groups. Conclusions: Diosmin can be administered for a protection of destructive effects of hepatic ischemia-reperfusion injury on pancreas both emergent and elective hepatic surgical operations in which possible ischemic periods were expected.