The effect of low incubation temperature on the embryonic development and larval hatching of the monogenean Diplectanum aequans, a gill parasite of sea bass Dicentrarchus labrax, was studied. Groups of eggs of D. aequans were incubated at 5°C for different time periods (from 24 h to 28 days) before incubation at room temperature (18°C). Percentages of hatched larvae, aborted larvae and undeveloped embryos were estimated in comparison with the control group. Results show that eggs of the parasite are able to survive at 5°C, with a percentage that decreases as the incubation time at 5°C increases, reducing the rate of larval hatching.
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Cecchini, S., Saroglia, M., Cognetti-Varriale, A. M., Terova, G., & Sabino, G. (2001). Effect of low environmental temperature on embryonic development and egg hatching of Diplectanum aequans (Monogenea, Diplectanidae) infecting European sea bass, Dicentrarchus labrax. Fish Pathology, 36(1), 33–34. https://doi.org/10.3147/jsfp.36.33