Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Abstract

Marek's disease virus (MDV), a highly transmissible cell-associated neuropathic oncogenic alphaherpes virus affecting poultry health, resulting in considerable economic losses in poultry industry worldwide. Until now MDV still emerging and re emerging causing great economic losses in chicken despite of intensive vaccination and management policy used in poultry farms. However, cytokines and its role in MD pathogenesis and immunity had been described by some workers under certain experimental conditions by using different MDV strains challenge, they need to be more clarified during the more progressive lymphoma transformation stage. The present study aimed to examine the transcriptional profiling of a panel of cytokines genes in the splenic tissues of special broiler Japanese chickens (70-80 days old) contracted natural infection with MDV despite of intensive care and vaccination policy adopted by HVT and CVI988/Rispene. SYBR Green-based, real-time (RT)-PCR protocol was used to quantitate cytokine mRNA in freshly collected spleen tissue of MDV infected and control chicken. Changes in the levels of spleen interleukins (IL) as IL-6, IL-10, IL-18 and IL-12P35, IL-4, interferon-gamma (IFN-γ) and inducible nitric oxide synthase (iNOS) mRNA was determined. Relative Messenger RNA (mRNA) expression levels of the above mentioned genes, and β-actin as a reference gene, were achieved. The results of quantitative real-time PCR (qPCR) assays using revealed significant up regulation in the expression levels of IL-6, IL-10, IL-18, and IL-12P35). The changes in the mRNA levels of IL-4, IFN-γ and inducible nitric oxide synthesase …